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Antibodies
Antibodies
Anti-HCN4 antibody [SHG 1E5]
Anti-HCN4 antibody [SHG 1E5] from Abcam
Be the first to write a review!
Citations:
(36)
Supplier Page
Supplier Page from
Abcam for
Anti-HCN4 antibody [SHG 1E5]
Product Specs
Company Info
Citations
Reviews
Product Specs
Item
Anti-HCN4 antibody [SHG 1E5]
Company
Abcam
Price
Pricing Info
Supplier Page
View Supplier Product Page
Catalog Number
ab32675
Antibody Type
Primary
Isotype
IgG1
Host
Rat
Clone
SHG 1E5
Clonality
Monoclonal
Applications
Flow Cytometry, Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections), Western blot
Quantity
100 UL
Conjugate/Tag
Unconjugated
Concentration
1 mg/ml
Research Area
Neuroscience
Gene Name
Hcn4
Reactivity
Human, Rat
Target
HCN4
Add to Compare List
Abcam
Discovery Drive
Cambridge Biomedical Campus
Cambridge
CB2 0AX
United Kingdom
Phone
: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600
Company Profile
Website:
http://www.abcam.com
Citations (36)
(1)
Using brain cell-type-specific protein interactomes to interpret neurodevelopmental genetic signals in schizophrenia
iScience
May 19, 2023
Hsu YH, Pintacuda G, Liu R, Nacu E, Kim A, Tsafou K, Petrossian N, Crotty W, Suh JM, Riseman J,
et al
.
ab133265
;RRID: AB_11158532
HCN1
,
Proteintech Group
,Cat#
55222-1-AP
;RRID: AB_11182929 HCN4,
Abcam
,Cat#
ab32675
;RRID: AB_732770 HNRNPA2B1,
Abcam
,Cat#
ab6102
;RRID: AB_305293
IGF2BP1
,
Proteintech Group
,Cat#
22803-1-AP
;RRID:
(2)
Loss of function of the nuclear envelope protein LEMD2 causes DNA damage-dependent cardiomyopathy
The Journal of clinical investigation
November 15, 2022
Caravia XM, Ramirez-Martinez A, Gan P, Wang F, McAnally JR, Xu L, Bassel-Duby R, Liu N, Olson EN.
MA5-12960
,
clone
13-11;
1:100
),
Ki67
(
Thermo Fisher Scientific
,
PA5-19462
,
1:200
), and HCN4 (
Abcam
,
ab32675
,
clone
SHG 1E5;
1:50
). Sections were subsequently washed with 0.01% Triton X-100 in PBS 3 times and
(3)
RA signaling pathway combined with Wnt signaling pathway regulates human-induced pluripotent stem cells (hiPSCs) differentiation to sinus node-like cells
Stem cell research & therapy
July 18, 2022
Yin L, Wang FY, Zhang W, Wang X, Tang YH, Wang T, Chen YT, Huang CX.
were transferred onto a nitrocellulose
membrane
, and incubated with primary antibodies against HCN4 (
ab32675
;
Abcam
, Cambridge, MA, USA),
TBX18
(
ab115262
;
Abcam
, Cambridge, MA, USA), ISL-1(abs132916; Abcam, Cambridge,
(4)
Deciphering cellular signals in adult mouse sinoatrial node cells
iScience
January 21, 2022
Reddy GR, Ren L, Thai PN, Caldwell JL, Zaccolo M, Bossuyt J, Ripplinger CM, Xiang YK, Nieves-Cintrón M, Chiamvimonvat N,
et al
.
antibodies were used to incubate cells overnight at 4°C: (1) monoclonal
rat
anti-HCN4 (
1:300
dilution;
Abcam
ab32675
) and (2) polyclonal
goat
anti-ACVI (
1:100
dilution;
Santa Cruz
sc68138
). Cells were washed with PBS
(5)
Arrhythmogenic Hearts in PKD2 Mutant Mice Are Characterized by Cardiac Fibrosis, Systolic, and Diastolic Dysfunctions
Frontiers in Cardiovascular Medicine
November 26, 2021
Farideh Amirrad, Rajasekharreddy Pala, Kiumars Shamloo, Brian S. Muntean, Surya M. Nauli
cells in the atrioventricular node, tissues were incubated with
rat
anti-HCN4 antibody (
1:1,000
; Cat#
ab32675
;
Abcam
, Inc.) overnight at 4°C in a humidified chamber followed with AlexaFluor-488
goat
anti-
rat
fluorescence
(6)
A novel transgenic Cre allele to label mouse cardiac conduction system
Developmental Biology
October 1, 2021
Peter C. Kahr, Ge Tao, Zachary A. Kadow, Matthew C. Hill, Min Zhang, Shuang Li, James F. Martin
imaged with a Nikon Eclipse upright microscope. For
immunofluorescent staining
, antibodies against Hcn4 (
ab32675
,
Abcam
, Cambridge, UK), cardiac Troponin T (cTnT) (ms-295, Thermo Scientific, Waltham, MA, US), Connexin
(7)
PDGFRα-Signaling Is Dispensable for the Development of the Sinoatrial Node After Its Fate Commitment
Frontiers in Cell and Developmental Biology
June 10, 2021
Xi Zheng, Fengjiao Wang, Xiaoxiao Hu, Hua Li, Zhen Guan, Yanding Zhang, Xuefeng Hu
Institutes of Health). Antibodies used for this study include anti-Pdgfrα (R&D, AF1062), anti-Hcn4 (
Abcam
,
ab32675
), anti-Nkx2.5 (
Santa Cruz
,
sc8697
), and antiGFP (
Abcam
,
ab13970
). 3D Reconstruction Images of consecutive
(8)
Synergistic effects of ivabradine and metformin in the treatment of concomitant chronic heart failure and diabetes mellitus by regulating the activity of the H19/miR-423-5p/HCN4 axis
Archives of Medical Science
March 26, 2021
Fang Zhang, Weijun Jiang, Yating Liu, Liu Li, Rui Wang, Yang Li
bovine serum albumin at room temperature for 1 h, incubated with 216 anti-HCN4 primary antibodies (
ab32675
,
Abcam
, Cambridge, MA) at 4oC overnight, 217 incubated with HRP-labeled IgG secondary antibodies (
ab6721
,
(9)
Conjugated activation of myocardial-specific transcription of Gja5 by a pair of Nkx2-5-Shox2 co-responsive elements
Developmental Biology
September 1, 2020
Tianfang Yang, Zhen Huang, Hua Li, Linyan Wang, YiPing Chen
described previously (Li et al., 2019). The primary antibodies used in this study were: anti-Hcn4 (
ab32675
,
Abcam
;
1:200
), anti-Nkx2–5 (
AF2444
,
Novus Biologicals
;
1:200
), anti-Cx40 (Cx40-A; Alpha Diagnostic
(10)
ZO-1 Regulates Intercalated Disc Composition and Atrioventricular Node Conduction
Circulation research
July 3, 2020
Dai W, Nadadur RD, Brennan JA, Smith HL, Shen KM, Gadek M, Laforest B, Wang M, Gemel J, Li Y,
et al
.
Catalog #
37–8900
), β-catenin (
Cell Signaling Technology
, Catalog #
9562
), and HCN4 (
Abcam
, Catalog #
ab32675
) were used at
1:300
dilution.
Mouse
,
Rat
, and Rabbit HRP conjugated secondary antibodies were used at
(11)
Nkx2.5 insufficiency leads to atrial electrical remodeling through Wnt signaling in HL‑1 cells
Experimental and Therapeutic Medicine
October 25, 2019
Jingjing Chen, Shunen Xu, Wei Li, Lirong Wu, Long Wang, Yongkang Li, Wei Zhou
antibodies overnight at 4°C against
Nkx2.5
(
1:1,000
; cat. no.,
ab106923
;
Abcam
), HCN4 (
1:1,000
; cat. no.,
ab32675
;
Abcam
), Cx40 (
1:1,000
; cat. no.,
ab16585
;
Abcam
), SERCA2a (
1:500
; cat. no.,
ab2861
;
Abcam
), ryanodine
(12)
Adipose‑derived stem cells overexpressing SK4 calcium‑activated potassium channel generate biological pacemakers
International Journal of Molecular Medicine
October 16, 2019
Mei Yang, Qingyan Zhao, Hongyi Zhao, Ankang Yang, Fengyuan Wang, Xi Wang, Yanhong Tang, Congxin Huang
The
membranes
were incubated with primary antibodies against HCN4 (
rat
monoclonal antibody,
1:1,000
,
ab32675
,
Abcam
),
SK4
(rabbit anti-
rat
monoclonal antibody,
1:500
,
ab215990
,
Abcam
), phosphorylated extracellular
(13)
Transcriptomic Profiling of the Developing Cardiac Conduction System at Single-Cell Resolution
Circulation research
August 2, 2019
Goodyer WR, Beyersdorf BM, Paik DT, Tian L, Li G, Buikema JW, Chirikian O, Choi S, Venkatraman S, Adams EL,
et al
.
(Alpha Diagnostics, Cx40-A) at
1:100
dilution; Anti-mouse Hcn4
Rat
Monoclonal [SHG 1E5] antibody (
Abcam
,
ab32675
) at
1:75
dilution. The following secondaries were used at a
1:500
dilution: Donkey anti-goat IgG Alexa
(14)
Nkx2-5
defines a subpopulation of pacemaker cells and is essential for the physiological function of the sinoatrial node in mice
Development
March 18, 2019
Hua Li et al.
described previously (Ye et al., 2015b). The primary antibodies used in this study were: anti-Hcn4 (
ab32675
,
Abcam
;
1:500
), anti-Nkx2-5 (
sc-8697
,
Santa Cruz
;
1:500
), anti-Tbx3 (
sc-17871
,
Santa Cruz
;
1:500
), anti-Cx40
(15)
A new combination of transcription factors increases the harvesting efficiency of pacemaker‑like cells
Molecular Medicine Reports
March 6, 2019
Jian Zhang, Congxin Huang
potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 4 (HCN4;
1:1,000
; cat. no.
ab32675
;
Abcam
, Cambridge, MA, USA), connexin 43 (Cx43;
1:3,000
; cat. no.
ab11370
;
Abcam
) and connexin 45 (Cx45;
(16)
Insulin gene enhancer binding protein 1 induces adipose tissue‑derived stem cells to differentiate into pacemaker‑like cells
International Journal of Molecular Medicine
November 27, 2018
Jian Zhang, Mei Yang, An‑Kang Yang, Xi Wang, Yan‑Hong Tang, Qing‑Yan Zhao, Teng Wang, Yu‑Ting Chen, Cong‑Xin Huang
antibodies against hyperpolarization-activated cyclic nucleotide-gated cation channel (HCN)4 (cat. no.
ab32675
), connexin (Cx)43 (cat. no.
ab11370
; both
Abcam
, Cambridge, MA, USA) and Cx45 (cat. no. F5108; Affinity
(17)
Comparison of mouse brown and white adipose‑derived stem cell differentiation into pacemaker‑like cells induced by TBX18 transduction
Molecular medicine reports
May 1, 2018
Sun AJ, Qiao L, Huang C, Zhang X, Li YQ, Yang XQ.
no.
ab9465
) and hyperpolarization-activated cyclic nucleotide-gated channel 4 (HCN4;
rat
; cat. no.
ab32675
) primary antibodies were purchased from
Abcam
(Cambridge, MA, UK). β-actin primary antibody (WB0196;
(18)
Genetic targeting of Purkinje fibres by Sema3a-CreERT2
Scientific reports
February 5, 2018
Li Y, Tian X, Zhao H, He L, Zhang S, Huang X, Zhang H, Miquerol L, Zhou B.
ab6662
,
1:200
); TNNI3 (
Abcam
,
ab56357
,
1:100
); Cx40 (Alpha Diagnostic, Cx40-A,
1:100
); HCN4 (
Abcam
,
ab32675
,
1:100
); tyrosine hydroxylase (TH,
Millipore
,
AB152
,
1:100
); and Tuj1 (Covance, MMS-435P,
1:100
). The
(19)
Transcription factor TBX18 promotes adult rat bone mesenchymal stem cell differentiation to biological pacemaker cells
International Journal of Molecular Medicine
November 16, 2017
Yanjun Li, Mei Yang, Gege Zhang, Le Li, Bingjie Ye, Congxin Huang, Yanhong Tang
rat
anti-hyperpolarization-activated cyclic nucleotide-gated channel 4 (HCN4) monoclonal antibody (
ab32675
,
1:500
) and rabbit anti-connexin 43 (CX43) polyclonal antibody (
ab11370
,
1:2,000
) (all
Abcam
, Cambridge,
(20)
Involvement of Rictor/mTORC2 in cardiomyocyte differentiation of mouse embryonic stem cells
in vitro
International Journal of Biological Sciences
January 15, 2017
Bei Zheng, Jiadan Wang, Leilei Tang, Chao Tan, Zhe Zhao, Yi Xiao, Renshan Ge, Danyan Zhu
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Anti-HCN4 antibody [SHG 1E5] from Abcam
Supplier Page
View Supplier Product Page
Pricing Info
Product Specs
Company Info
Citations
Reviews
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